Yazd Journal of Dental Research

Introduction: The penetration of microorganisms and irritants into dentinal tubules necessitates utilizing some kinds of material to chemically clear the canal. Therefore, the present study aimed to compare the
anti-bacterial effect of Peranacid M2 with Sodium Hypochlorite and Chlorhexidine.

Methods: In this experimental-laboratory study, Muller-Hington medium was prepared containing 5% of sheep blood. After root canal preparation, the discs containing 5.25% Sodium Hypochlorite, 2% Chlorhexidine, and Pranacid M2 were exposed to Enterococcus Fecalis. Each disc was located on a plate with a 2 cm distance from another and then, the plates were incubated in 37 °C for 24 hours. After ending the incubation period, the average diameter of inhibition zone was measured around the discs (in millimeter). This procedure was repeated 10 times for every irrigator. The study data were analyzed via SPSS software (ver.17) applying ANOVA and Tukey HSD tests.

Results: A significant difference was observed in the diameter of antimicrobial zone of the three irrigating solutions (P=0.0001). The most anti-bacterial effects were observed in 5.25% Sodium Hypochlorite (27.3±2.05) and then 2% Chlorhexidine (18.9±1.28). In addition, the least effect was reported in Peranacid M2(10.3±1.41).

Conclusion: Taking the limitations of an in vitro study into account, Peranasid M2 seems to possess less antibacterial effect compared to 5.25%  Sodium Hypochlorite and 2% Chlorhexidine.